rna-seq analysis – Christian Health Association of Ghana, original RealCycler CHAG

cryopreservation techniques as a conservation method of donkey germplasm

Megan Detergent, RCCS February 12, 2022February 12, 2022phosphate 500 mg, phosphate acquisition, phosphate anion, phosphate binders, phosphate buffer, phosphate buffered saline, phosphate calcium, phosphate charge, phosphate fertilizer, phosphate formula, phosphate glass, phosphate group, phosphate ion, phosphate ion formula, phosphate levels, phosphate normal range, phosphate repletion, phosphate rock mining jobs plano, phosphate sodium, phosphate symbol, phosphate tricalcique, phosphate vs phosphorus, phosphate 翻译, phosphate中文, rna base pairs, rna bases, rna definition, rna function, rna medical, rna polymerase, rna reset, rna sequencing, rna splicing, rna structure, rna transcription, rna vaccine, rna virus, rna vs dna, rna-seq, rna-seq analysis, rnai, rnalater, rnao, rnascope, rnase, rnaseq, rnation login, rnav 0 Comment

Ejaculates of three male donkeys were used (n= 18; six ejaculates per donkey; six repetitions), collected by the artificial vagina method. To remove the seminal plasma (SP), the ejaculates were split and submitted to filtration or centrifugation methods. To assess the freezing method, each fraction were submitted to the automated system or the conventional system, and groups were formed: automated centrifuge (AC), automated filtrate (AF), conventional centrifuge (CC) and conventional filtrate (CF).

After thawing (37°C/30 s), were analyzed the sperm kinetic parameters, integrity and functionality of the plasma membrane and mitochondrial membrane potential. Highest sperm concentration (P<0.05) was observed in the filtrate groups; the CF group presented lower (P<0.05) progressive motility and curvilinear velocity compared to the other groups; no difference was observed (P>0.05) among the groups for the membrane integrity and functionality, and mitochondrial membrane potential. Thus, centrifugation is the most indicated technique to remove donkey seminal plasma and the automated and conventional freezing methods can be used in donkey semen conservation.

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Nitrogen resource recovery from mature leachate via heat extraction technology: An engineering project application

A large pool of ammonia in mature leachate is challenging to treat with a membrane bioreactor system to meet the discharge Standard for Pollution Control on the Landfill Site of Municipal Solid Waste in China (GB 16889-2008) without external carbon source addition. In this study, an engineering leachate treatment project with a scale of 2,000 m³/d was operated to evaluate the ammonia heat extraction system (AHES), which contains preheat, decomposition, steam-stripping, ammonia recovery, and centrifuge dewatering.

The operation results showed that NH₃-N concentrations of raw leachate and treated effluent from an ammonia heat extraction system (AHES) were 1,305-2,485 mg/L and 207-541 mg/L, respectively. The ratio of COD/NH₃-N increased from 1.40-1.84 to 7.69-28.00. Nitrogen was recovered in the form of NH₄HCO₃ by the ammonia recovery tower with the introduction of CO₂, wherein the mature leachate can offer 37% CO₂ consumption. The unit consumptions of steam and power were 8.0% and 2.66 kWh/m³ respectively, and the total operation cost of AHES was 2.06 USD per cubic metre of leachate. These results confirm that heat extraction is an efficient and cost-effective technology for the recovery of nitrogen resource from mature leachate.

Field Determination of Phosphate in Environmental Water by Using a Hand-Powered Paper Centrifuge for Preconcentration and Digital Image Colorimetric Sensing

Phosphate concentration in natural water has been used as a water quality indicator, as it is one of the major nutrients for aquatic plants. However, the traditional phosphomolybdenum blue (PMB) method has limited sensitivity for visual or camera-based detection, leading to underestimation of the phosphate concentration. We present an ultralow-cost, rapid field preconcentration and digital image colorimetric sensing of low-concentration phosphate method for water analysis. A novel hand-powered paper centrifuge (paperfuge) is used for sample preparation and preconcentration.

This paperfuge is made of two circular paper discs and a string. Six centrifuge tubes (CTs) originally used as glue dispensing tips with a sample capacity of ∼230 μL, are loaded on the paperfuge. After sampling, phosphate in the water sample is reacted to form PMB. Then, the reacted sample is drawn into a CT using an autopipette before the CT bottom is sealed by glue. After Oasis HLB sorbents are added through the top of the CT, the CT top is also sealed with glue.

The HLB sorbents adsorb PMB and are accumulated in the CT tip through centrifugation. The CT tips are cut and analyzed with the ImageJ software. It was found that the blue color intensity of sorbents is in a linear relationship to the phosphate concentration, with a linear range of 0-5 μM (r ² = 0.9921) and limit of detection of 0.19 μM. In addition, this method has been applied to in-field water analysis. The results are in agreement with the standard PMB method.

Simultaneous determination of 36 hypotensive drugs in fingerprints by ultra performance liquid chromatography-triple quadrupole composite linear ion trap mass spectrometry

Fingerprints contain important information such as the ingredients ingested by the donor. By analyzing the characteristic components in fingerprints, the donor can be characterized, which would provide insights for investigation of a given case. This approach can also be used in the qualitative monitoring of drug intake. Therefore, the examination of hypotensive drugs in fingerprints has significant value in practical application. This study established a method based on ultra performance liquid chromatography-triple quadrupole composite linear ion trap mass spectrometry (UPLC-Q-TRAP/MS) for the simultaneous determination of 36 hypotensive drugs in fingerprints.

The pre-treatment method was based on protein precipitation. A 3×3 cm filter paper was cut into pieces and placed in a 2 mL plastic centrifuge tube after fingerprint collection. Then, 0.50 mL methanol was added, followed by vortex mixing for 1 min and ultrasonic oscillation for 3 min. The filter paper was centrifuged at 12000 r/min for 5 min, and the supernatant was withdrawn for sample analysis.

An ACQUITY UPLC BEH C18 chromatographic column (100 mm×3.0 mm, 1.7 μm) was selected, with 0.01% aqueous formic acid and methanol as mobile phases for gradient elution. MS analysis involved scheduled multiple reaction monitoring-information dependent acquisition-enhanced product ion (SMRM-IDA-EPI) scanning. This method could be used to retrieve library researching during high-sensitivity analysis, which could increase the accuracy of qualitative results.

The calibration curves showed good linearity in the range of 0.05-50.00 ng/fingerprint, with correlation coefficients (r) greater than 0.99 for all 36 analytes. The limits of detection and limits of quantification of the 36 hypotensive drugs were 0.001-0.045 ng/fingerprint and 0.002-0.050 ng/fingerprint, respectively. At spiked levels of 0.25, 2.50, 25.00 ng/fingerprint, the matrix effects, recoveries, intra-day precisions, and inter-day precisions of the 36 hypotensive drugs were 79.0%-119.2%, 79.3%-116.2%, 0.2%-18.3%, and 1.6%-19.1%, respectively.

This method was used to detect hypotensive drugs in the fingerprints of 87 hypertensive patients, and hypotensive drug intakes were accurately detected in most cases. The established method is operationally simple, with high sensitivity and good selectivity, and it is suitable for screening and testing hypotensive drugs in fingerprints.

Pyridine-functional diblock copolymer nanoparticles synthesized via RAFT-mediated polymerization-induced self-assembly: effect of solution pH

Polymerization-induced self-assembly (PISA) via reversible addition-fragmentation chain transfer (RAFT) polymerization has become widely recognized as a versatile and efficient strategy to prepare complex block copolymer nanoparticles with controlled morphology, size, and surface functionality. In this article, we report the preparation of cationic sterically-stabilized poly(2-vinylpyridine)-poly(benzyl methacrylate) (P2VP-PBzMA) diblock copolymer nanoparticles via RAFT-mediated PISA under aqueous emulsion polymerization conditions.

It is demonstrated that the solution pH during PISA has a dramatic effect on the resulting P2VP-PBzMA nanoparticles, as judged by dynamic light scattering (DLS), disc centrifuge photosedimentometry (DCP) and transmission electron microscopy (TEM).

Varying the solution pH results in the P2VP stabilizer having different solubilities due to protonation/deprotonation of the pyridine groups. This allows P2VP-PBzMA nanoparticles with tunable diameters to be prepared by altering the DP of the stabilizer (P2VP) and/or core-forming block (PBzMA), or simply by changing the solution pH for a fixed copolymer composition.

For example, P2VP-PBzMA nanoparticles with larger diameters can be obtained at higher solution pH as the protonation degree of the P2VP stabilizer has a large effect on both the aggregation of polymer chains during the PISA process, and the resulting behavior of the diblock copolymer nanoparticles. Changing the dispersion pH post-polymerization has a relatively limited effect on particle diameter. Furthermore, aqueous electrophoresis studies indicate that these P2VP-PBzMA nanoparticles had good colloidal stability and high cationic charge (>30 mV) below pH 5 and can be dispersed readily over a wide pH range.

Axygen Gel Documentation System
GD-1000	Scientific Laboratory Supplies	EACH	5947.38 EUR
AXYGEN® GEL DOCUMENTATION SYSTEM BL
GDBL-1000	CORNING	1/pk	9158.4 EUR
UVP GelSolo M-20V Gel Documentation System
AJ849-97-0934-02	Westburg	each	17440 EUR
UVP GelSolo LM-20 Gel Documentation System
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UVP GelSolo LM-26 Gel Documentation System
AJ849-97-0938-02	Westburg	each	18475.5 EUR
UVP GelSolo LMS-20 Gel Documentation System
AJ849-97-0939-02	Westburg	each	19238.5 EUR
UVP GelSolo LMS-26 Gel Documentation System
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Dual LED Transilluminator Gel Documentation System
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Dual LED Transilluminator Gel Documentation System
abx795001-5nmol	Abbexa	5 nmol	962.5 EUR
PHOTO DOCUMENTATION SYSTEM
VLBPCX4-20M	Consort	ea	10300 EUR
photo documentation system
VLBPCX4-20MX	Consort	ea	8128.8 EUR
photo documentation system
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photo documentation system
VLDP17-26M	Consort	ea	9751.2 EUR
photo documentation system
VLDP17-26MX	Consort	ea	10156.8 EUR
AXYGEN® GEL DOCUMENTATION SYSTEM BLUE LIGHT CONVERSION SCREEN FOR GD-1000
GD-BLCS	CORNING	1/pk	840 EUR
Mini-6 Gel Documentation Sys - EACH
ELE6580	Scientific Laboratory Supplies	EACH	29938.95 EUR
Mini-9 Gel Documentation Sys - EACH
ELE6582	Scientific Laboratory Supplies	EACH	31309.2 EUR
AXYGEN® GEL DOCUMENTATION SYSTEM WHITE LIGHT CONVERSION SCREEN FOR GD-1000 AND GDBL-1000
GD-WLCS	CORNING	1/pk	427.2 EUR
IQ/OQ Documentation for Esco CO2 incubators - EACH
INC2088	Scientific Laboratory Supplies	EACH	1263.17 EUR
IQ/OQ documentation for the C40 (incl. N-4080-HNF)
IMN-40-QHN	Westburg	each	1798.5 EUR
IQ/OQ documentation for the NP80 (incl. N-4080-HNF and N-568-S)
IMN-80-QHN	Westburg	each	2125.5 EUR
Freezer Accessory IQ/OQ Documentation for Esco ULT freezers - EACH
FRE2814	Scientific Laboratory Supplies	EACH	931.22 EUR
21 CFR Part 11 software for the N60 incl. IQ/OQ documentation and solution
IMCFR21-Q-60	Westburg	each	4360 EUR
IQ/OQ documentation for the NanoPhotometer N60 incl. Standard Solution
IMN-60-Q	Westburg	each	1308 EUR
21 CFR Part 11 software package for the NP40 incl. IQ/OQ documentation and parts
IMCFR21-QHN-40	Westburg	each	4905 EUR

A very long-acting IL-15: implications for the immunotherapy of cancer

Megan RCCS, RNA February 2, 2022polyclonal, polyclonal antibodies definition, polyclonal antibodies regeneron, polyclonal antibody production, polyclonal b cells, polyclonal gammopathy, polyclonal gammopathy treatment, polyclonal hypergammaglobulinemia, polyclonal immunoglobulin, polyclonal increase, polyclonal increase in immunoglobulins, polyclonal vs monoclonal, polyclonal vs monoclonal antibody, rna base pairs, rna bases, rna definition, rna function, rna interference, rna medical, rna polymerase, rna polymerase function, rna reset, rna sequencing, rna splicing, rna structure, rna transcription, rna vaccine, rna virus, rna vs dna, rna-seq analysis, rnai, rnao, rnascope, rnase, rnaseq, rnation login, rnav 0 Comment

Background: Interleukin-15 (IL-15) is an important cytokine necessary for proliferation and maintenance of natural killer (NK) and CD8⁺ T cells, and with great promise as an immuno-oncology therapeutic. However, IL-15 has a very short half-life and a single administration does not provide the sustained exposure required for optimal stimulation of target immune cells. The purpose of this work was to develop a very long-acting prodrug that would maintain IL-15 within a narrow therapeutic window for long periods-similar to a continuous infusion.

Methods: We prepared and characterized hydrogel microspheres (MS) covalently attached to IL-15 (MS~IL-15) by a releasable linker. The pharmacokinetics and pharmacodynamics of MS~IL-15 were determined in C57BL/6J mice. The antitumor activity of MS~IL-15 as a single agent, and in combination with a suitable therapeutic antibody, was tested in a CD8⁺ T cell-driven bilateral transgenic adenocarcinoma mouse prostate (TRAMP)-C2 model of prostatic cancer and a NK cell-driven mouse xenograft model of human ATL (MET-1) murine model of adult T-cell leukemia.

Results: On subcutaneous administration to mice, the cytokine released from the depot maintained a long half-life of about 168 hours over the first 5 days, followed by an abrupt decrease to about ~30 hours in accordance with the development of a cytokine sink. A single injection of MS~IL-15 caused remarkably prolonged expansions of NK and ɣδ T cells for 2 weeks, and CD44^hiCD8⁺ T cells for 4 weeks. In the NK cell-driven MET-1 murine model of adult T-cell leukemia, single-agent MS~IL-15_{50 μg} or anti-CCR4 provided modest increases in survival, but a combination-through antibody-depedent cellular cytotoxicity (ADCC)-significantly extended survival. In a CD8⁺ T cell-driven bilateral TRAMP-C2 model of prostatic cancer joplink Rubisco Mouse Polyclonal Antibody, single agent subcutaneous MS~IL-15 or unilateral intratumoral agonistic anti-CD40 showed modest growth inhibition, but the combination exhibited potent, prolonged bilateral antitumor activity.

Conclusions: Our results show MS~IL-15 provides a very long-acting IL-15 with low C_max that elicits prolonged expansion of target immune cells and high anticancer activity, especially when administered in combination with a suitable immuno-oncology agent.

Indirect signal amplification strategy with a universal probe-based lateral flow immunoassay for the rapid quantitative detection of fumonisin B1

Fumonisin B1 (FB1) is a serious threat to the health of humans and animals. Herein, a lateral flow immunoassay based on universal detection probes (goat anti-mouse IgG@Eu) that could combine with any mouse monoclonal antibody was applied to detect FB1 in corn and feed. Compared with that based on direct monoclonal antibody labeling, this assay maintained bioactivity and saved consumption of monoclonal antibodies with the indirect signal amplification effect.

The results indicated that this assay had higher sensitivity with a limit of detection (LOD) of 0.025 and 0.097 ng mL^-1 (0.50 and 1.94 ng g^-1 based on sample weight) in corn and feed, respectively. The detection range was about 1-50 ng mL^-1 (20-1000 ng g^-1 based on sample weight). In addition, the evaluation proved that it had good specificity, accuracy, precision, and applicability, and thus was suitable for the rapid and low-cost detection of fumonisin B1.

TSH-TSHR axis promotes tumor immune evasion

Background: Hormones are identified as key biological variables in tumor immunity. However, previous researches mainly focused on the immune effect of steroid hormones, while the roles that thyroid-stimulating hormone (TSH) played in the antitumor response were far from clear.

Methods: The source of TSH was determined using single-cell transcriptomic, histologic, quantitative PCR, and ELISA analysis. The influence of TSH on tumor proliferation, invasion, and immune evasion was evaluated in multiple cell lines of thyroid cancer, glioma, and breast cancer. Then transcriptomic sequencing and cellular experiments were used to identify signaling pathways. TSH receptor (TSHR) inhibitor was injected into homograft mouse tumor models with or without anti-programmed cell death protein-1 antibody.

Results: Monocyte-derived dendritic cells (moDCs) highly expressed TSHα and TSHβ2 and were the primary source of TSH in the tumor microenvironment. TSH released by moDCs promoted proliferation and invasion of tumors with high TSHR expressions, such as thyroid cancers and glioma. TSH also induced tumor programmed death-ligand 1 (PD-L1) expression through the TSHR-AC-PKA-JNK-c-JUN pathway. TSHR inhibitors reversed tumor immune evasion by inhibiting PD-L1 expression in tumor and myeloid cells and enhancing Teff activation.

Conclusions: TSH-TSHR axis promotes tumor evasion in thyroid cancers and glioma. TSH suppression therapy is an effective therapeutic strategy for combination in immune checkpoint blockades.

A universal in silico V(D)J recombination strategy for developing humanized monoclonal antibodies

Background: Humanization of mouse monoclonal antibodies (mAbs) is crucial for reducing their immunogenicity in humans. However, humanized mAbs often lose their binding affinities. Therefore, an in silico humanization method that can prevent the loss of the binding affinity of mAbs is needed.

Methods: We developed an in silico V(D)J recombination platform in which we used V(D)J human germline gene sequences to design five humanized candidates of anti-tumor necrosis factor (TNF)-α mAbs (C1-C5) by using different human germline templates. The candidates were subjected to molecular dynamics simulation. In addition, the structural similarities of their complementarity-determining regions (CDRs) to those of original mouse mAbs were estimated to derive the weighted interatomic root mean squared deviation (wRMSD_i) value. Subsequently, the correlation of the derived wRMSDi value with the half maximal effective concentration (EC50) and the binding affinity (K_D) of the humanized anti-TNF-α candidates was examined. To confirm whether our in silico estimation method can be used for other humanized mAbs, we tested our method using the anti-epidermal growth factor receptor (EGFR) a4.6.1, anti-glypican-3 (GPC3) YP9.1 and anti-α4β1 integrin HP1/2L mAbs.

Results: The R² value for the correlation between the wRMSD_i and log(EC50) of the recombinant Remicade and those of the humanized anti-TNF-α candidates was 0.901, and the R² value for the correlation between wRMSD_i and log(K_D) was 0.9921. The results indicated that our in silico V(D)J recombination platform could predict the binding affinity of humanized candidates and successfully identify the high-affinity humanized anti-TNF-α antibody (Ab) C1 with a binding affinity similar to that of the parental chimeric mAb (5.13 × 10^-10). For the anti-EGFR a4.6.1, anti-GPC3 YP9.1, and anti-α4β1 integrin HP1/2L mAbs, the wRMSD_i and log(EC50) exhibited strong correlations (R² = 0.9908, 0.9999, and 0.8907, respectively).

Conclusions: Our in silico V(D)J recombination platform can facilitate the development of humanized mAbs with low immunogenicity and high binding affinities. This platform can directly transform numerous mAbs with therapeutic potential to humanized or even human therapeutic Abs for clinical use.

Rubisco Mouse Polyclonal Antibody
100ul	124 EUR
Rubisco Mouse Polyclonal Antibody
50ul	74 EUR
Rubisco Mouse Polyclonal Antibody(Large Chain)
100ul	319 EUR
Rubisco Mouse Polyclonal Antibody(Large Chain)
100ul	302.4 EUR
Rubisco Mouse Polyclonal Antibody(Large Chain)
50ul	224.4 EUR
Rubisco Mouse Polyclonal Antibody(Large Chain)
each	402 EUR
Rubisco Mouse Polyclonal Antibody(Large Chain)
100ul	379.2 EUR
Rubisco Mouse Polyclonal Antibody(Large Chain)
100μg/100μl	255 EUR
Rubisco Mouse Polyclonal Antibody(Large Chain)
100μg/100μl	225 EUR
Rubisco Mouse Polyclonal Antibody(Large Chain)
100μg/100μl	225 EUR

Nitrogen resource recovery from mature leachate via heat extraction technology: An engineering project application

Field Determination of Phosphate in Environmental Water by Using a Hand-Powered Paper Centrifuge for Preconcentration and Digital Image Colorimetric Sensing

Simultaneous determination of 36 hypotensive drugs in fingerprints by ultra performance liquid chromatography-triple quadrupole composite linear ion trap mass spectrometry

Pyridine-functional diblock copolymer nanoparticles synthesized via RAFT-mediated polymerization-induced self-assembly: effect of solution pH

Axygen Gel Documentation System

AXYGEN® GEL DOCUMENTATION SYSTEM BL

UVP GelSolo M-20V Gel Documentation System

UVP GelSolo LM-20 Gel Documentation System

UVP GelSolo LM-26 Gel Documentation System

UVP GelSolo LMS-20 Gel Documentation System

UVP GelSolo LMS-26 Gel Documentation System

Dual LED Transilluminator Gel Documentation System

Dual LED Transilluminator Gel Documentation System

PHOTO DOCUMENTATION SYSTEM

photo documentation system

photo documentation system

photo documentation system

photo documentation system

AXYGEN® GEL DOCUMENTATION SYSTEM BLUE LIGHT CONVERSION SCREEN FOR GD-1000

Mini-6 Gel Documentation Sys - EACH

Mini-9 Gel Documentation Sys - EACH

AXYGEN® GEL DOCUMENTATION SYSTEM WHITE LIGHT CONVERSION SCREEN FOR GD-1000 AND GDBL-1000

IQ/OQ Documentation for Esco CO2 incubators - EACH

IQ/OQ documentation for the C40 (incl. N-4080-HNF)

IQ/OQ documentation for the NP80 (incl. N-4080-HNF and N-568-S)

Freezer Accessory IQ/OQ Documentation for Esco ULT freezers - EACH

21 CFR Part 11 software for the N60 incl. IQ/OQ documentation and solution

IQ/OQ documentation for the NanoPhotometer N60 incl. Standard Solution

21 CFR Part 11 software package for the NP40 incl. IQ/OQ documentation and parts

Indirect signal amplification strategy with a universal probe-based lateral flow immunoassay for the rapid quantitative detection of fumonisin B1

TSH-TSHR axis promotes tumor immune evasion

A universal in silico V(D)J recombination strategy for developing humanized monoclonal antibodies

Rubisco Mouse Polyclonal Antibody

Rubisco Mouse Polyclonal Antibody

Rubisco Mouse Polyclonal Antibody(Large Chain)

Rubisco Mouse Polyclonal Antibody(Large Chain)

Rubisco Mouse Polyclonal Antibody(Large Chain)

Rubisco Mouse Polyclonal Antibody(Large Chain)

Rubisco Mouse Polyclonal Antibody(Large Chain)

Rubisco Mouse Polyclonal Antibody(Large Chain)

Rubisco Mouse Polyclonal Antibody(Large Chain)

Rubisco Mouse Polyclonal Antibody(Large Chain)