Development during COVID-19 pandemic: the role of coronavirus testing and functional labs

 This study analyzes the money supply reaction to the COVID-19 pandemic using a cross-sectional panel of 115 countries. The study used robust least square regression and innovation accounting techniques to get sound parameter estimates.
The results show that COVID-19 infected cases are the main contributing factor that obstructs financial activities and decrease money supply. In contrast, an increasing number of recovered cases and COVID-19 testing capabilities gave investors confidence to increase stock trade across countries. The overall forecast trend shows that COVID-19 infected cases and recovered cases followed the U-shaped trend, while COVID-19 critical cases and reported deaths showed a decreasing trend.
Finally, the money supply and testing capacity show a positive trend over a period. The study concludes that financial development can be expanded by increasing the testing capacity of Gentaur Cellulose Stoppers and functional labs to identify suspected coronavirus cases globally.

Recovering metal(loids) and rare earth elements from closed landfill sites without excavation: Leachate recirculation opportunities and challenges

Metal (loids) and Rare Earth Elements (REE) (‘metals’) are used in a wide range of products, and therefore, the improvement of expectations for everyday comforts with demand continues to grow. Metal-bearing wastes are a secondary source of raw material that can meet this demand by providing a previously unconsidered low impact supply source. Total annual leachate production is 1,056,716 m3. Therefore, landfill leachate emerges as a significant potential resource as it contains high concentrations of metals. However, realising a profitable return on investment for leachate processing is a challenge due to relatively low recovery rates of approximately 0.02% of total heavy metals in a landfill being leached out in 30 years.
Variation within the multi-element value and the effect of other chemicals in these complex mixtures. There is a need to better understand the mechanisms and potential applicability of extraction methods for optimising metals recovery from leachate. This paper addresses this need by providing a systematic review of the critical factors and environmental conditions that influence the behaviour of metals within the landfilled waste.
The paper provides a synthesis of how the factors and conditions may affect leachate recirculation efficiency for recovery in the context of a range of opportunities and challenges facing circular economy practitioners. To approach feasibility metal recovery economically from landfill leachate without energy-intensive and environmentally destructive, future research actions need to be initiated in lab-based and later on semi-pilot to pilot studies, which the review can help achieve the challenges.

Amino acid pattern of rumen microorganisms in cattle fed mixed diets-An update

Rumen microorganisms turn small N-containing compounds into amino acids (AA) and contribute considerably to the supply of AA absorbed from the small intestine. Previous studies summarized the literature on microbial AA patterns, most recently in 2017 (Sok et al. Journal of Dairy Science, 100, 5241-5249). The present study intended to identify the microbial AA pattern typical when feeding Central European diets and a maximum proportion of concentrate (PCO; dry matter (DM) basis) of 0.60.
Data sets were created from the literature for liquid (LAB)- and particle (PAB)-associated bacteria, total bacteria and protozoa, including 16, 9, 27 and 8 studies and 36, 21, 60 and 18 diets respectively. Because the only differences detected between LAB and PAB were slightly higher Phe and lower Thr percentages in PAB (p < 0.05), results for bacteria were pooled. A further data set evaluated AA-N (AAN) as a proportion of total N in microbial fractions and a final data set estimated protozoal contributions to total microbial N (TMN) flow to the duodenum, which were used to calculate weighted TMN AA patterns.
Protozoa showed higher Lys, Asp, Glu, Ile and Phe and lower Ala, Arg, Gly, Met, Ser, Thr and Val proportions than bacteria (p < 0.05). The AAN percentage of total N in bacteria and protozoa showed large, unexplained variations, averaging 79.0% and 70.6% (p > 0.05) respectively.
Estimation of protozoal contribution to TMN resulted in a cattle-specific mixed model including PCO and DM intake (DMI) per unit of metabolic body size (kg0.75 ) as fixed effects (RMSE = 3.77). With moderate PCO and DMI between 80 and 180 g/kg0.75 , which corresponds to a DMI of approximately 10 to 25 kg in a cow with 650 kg body weight, protozoal contribution ranged between 9% and 26% of TMN. Within this range, the estimated protozoal contribution to TMN resulted in minor effects on the total microbial AA pattern.

Sustainable phosphorus management in soil using bone apatite

Soil fertility and phosphorus management by bone apatite amendment are receiving increasing attention, yet further research is needed to integrate the physicochemical and mineralogical transformation of bone apatite and their impact on the supply and storage of phosphorus in soil. This study has examined bone transformation in the field over a span of 10-years using a set of synchrotron-based microscopic and spectroscopic techniques. Transmission X-ray microscopy (TXM) observations reveal the in-situ deterioration of bone osteocyte-canaliculi system and sub-micron microbial tunneling within a year. Extensive organic decomposition, secondary mineral formation and re-mineralization of apatite are evident from the 3rd year.
The relative ratio of (v1 + v3) PO43- to v3 CO32- and to amide I increase, and the v3c PO43- peak exhibits a blue-shift in less than 3 years. The carbonate substitution of bone hydroxyapatite (HAp) to AB-type CHAp, and phosphate crystallographic rearrangement become apparent after 10 years’ aging. The overall CO32- peak absorbance increases over time, contributing to a higher acid susceptibility in the aged bone.
The X-ray Photoelectron Spectroscopy (XPS) binding energies for Ca (2p), P (2p) and O (1s) exhibit a red-shift after 1 year because of organo-mineral interplay and a blue-shift starting from the 3rd year as a result of the de-coupling of mineral and organic components. Nutrient supply to soil occurs within months via organo-mineral decoupling and demineralization.
More phosphorus has been released from the bones and enriched in the associated and adjacent soils over time. Lab incubation studies reveal prominent secondary mineral formation via re-precipitation at a pH similar to that in soil, which are highly amorphous and carbonate substituted and prone to further dissolution in an acidic environment. Our high-resolution observations reveal a stage-dependent microbial decomposition, phosphorus dissolution and immobilization via secondary mineral formation over time. The active cycling of phosphorus within the bone and its interplay with adjacent soil account for a sustainable supply and storage of phosphorus nutrients.

Chemical characterization of dissolved organic matter as disinfection byproduct precursors by UV/fluorescence and ESI FT-ICR MS after smoldering combustion of leaf needles and woody trunks of pine (Pinus jeffreyi)

Forested land plays an essential role in water supply across the United States (US). Smoldering commonly existing in wildfires contributes significantly to biomass consumption and gas emission, but its influence on source water quality has been rarely studied. Here, we investigated the impact of smoldering temperature (i.e., no burn, 250, 400, and 600 °C) on the nutrients, elements, and dissolved organic matter (DOM) of water extracts from the residues of the leaf needles and woody trunks of pine (Pinus jeffreyi) under the lab-simulated smoldering fire. Results showed the increase of pH and the yields of the dominated exchangeable cations of K+ and Mg2+, P, PO43--P, and SO42- with increasing temperature increasing from 250 to 600 °C, whereas significant decreases in the fraction of dissolved organic C in residue C with increasing temperature and the yields of dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) after burnings.
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) presented consistent results with UV/fluorescence, suggesting that the unburned materials contained more biodegradable tyrosine/tryptophan/soluble microbial byproduct-like compounds with high molecular weight (MW), whereas the 600 °C-smoldering materials composed of more aromatic, humified, fulvic/humic acid-like, and oxidized compounds with a potentially high density of C=C bonds had less reactivity in forming trihalomethanes (THMs) and haloacetonitriles (HANs). Our study indicates the smoldering-dominated prescribed fire as a potential forest management strategy for reducing biomass fuel and disinfection byproducts (DBPs) precursors in source water from forested lands.

Turnover Stoppers 6.5mm

STO6300 Scientific Laboratory Supplies PK10 14.35 EUR

Turnover Stoppers 8mm

STO6302 Scientific Laboratory Supplies PK10 12.78 EUR

Turnover Stoppers 9.5mm

STO6304 Scientific Laboratory Supplies PK10 15.63 EUR

Turnover Stoppers 12.5mm

STO6308 Scientific Laboratory Supplies PK10 14.35 EUR

Turnover Stoppers 17.5mm

STO6314 Scientific Laboratory Supplies PK10 21.31 EUR

Turnover Stoppers 20.5mm

STO6318 Scientific Laboratory Supplies PK10 29.9 EUR

Turnover Stoppers 25.5mm

STO6324 Scientific Laboratory Supplies PK10 41.26 EUR

Turnover Stoppers 11mm

STO6306 Scientific Laboratory Supplies PK10 15.63 EUR

Turnover Stoppers 14mm

STO6310 Scientific Laboratory Supplies PK10 14.35 EUR

Turnover Stoppers 16mm

STO6312 Scientific Laboratory Supplies PK10 17.05 EUR

Turnover Stoppers 19mm

STO6316 Scientific Laboratory Supplies PK10 24.81 EUR

Turnover Stoppers 22mm

STO6320 Scientific Laboratory Supplies PK10 38.38 EUR

Turnover Stoppers 24mm

STO6322 Scientific Laboratory Supplies PK10 39.82 EUR

Stoppers B12/21 PE STOPPER RED INSERT

TRF592 Scientific Laboratory Supplies PK10 24 EUR

Stoppers B19/26 PE STOPPER RED INSERT

TRF595 Scientific Laboratory Supplies PK10 27.6 EUR

Lock Stoppers Butyrometer GERBAL F

BUT1010 Scientific Laboratory Supplies PK10 92.4 EUR

Methyl cellulose

GC0717-1KG Glentham Life Sciences 1 kg 206.4 EUR

Methyl cellulose

HY-125861 MedChemExpress 500mg 129.6 EUR

Methyl cellulose

GC0717-1 Glentham Life Sciences 1 125.1 EUR

Methyl cellulose

GC0717-100 Glentham Life Sciences 100 20.3 EUR

Methyl cellulose

GC0717-250 Glentham Life Sciences 250 40.7 EUR

Methyl cellulose

GC0717-500 Glentham Life Sciences 500 71.1 EUR

Ethyl cellulose, 100 cP

GC4298-500G Glentham Life Sciences 500 g 180 EUR

Ethyl cellulose, 100 cP

GC4298-100 Glentham Life Sciences 100 63.3 EUR

Ethyl cellulose, 100 cP

GC4298-500 Glentham Life Sciences 500 197.7 EUR

Hydroxyethyl cellulose

20-abx184119 Abbexa
  • 260.40 EUR
  • 360.00 EUR
  • 100 g
  • 500 g

Hydroxyethyl cellulose

HY-B2221B MedChemExpress 500mg 129.6 EUR

Hydroxypropyl Cellulose

B1771-50 ApexBio 50 mg 153.6 EUR

 

A powerful next-generation solution to tackle hepatocellular carcinoma

Meanwhile, current therapeutic modalities such as liver resection and transplantation are only effective for resolving early-stage HCC. Hence, alternative approaches are required to improve detection and enhance the efficacy of current treatment options. Nanotheranostic platforms, which utilize biocompatible nanoparticles to perform both diagnostics and targeted delivery, has been considered a potential approach for cancer management in the past few decades.
Advancement of nanomaterials and biomedical engineering techniques has led to rapid expansion of the nanotheranostics field, allowing for more sensitive and specific diagnosis, real-time monitoring of drug delivery, and enhanced treatment efficacies across various malignancies. The focus of this review is on the applications of nanotheranostics for HCC.
The review first explores the current epidemiology and the commonly encountered obstacles in HCC diagnosis and treatment. It then presents the current technological and functional advancements in nanotheranostic technology for cancer in general, and then specifically explores the use of nanotheranostic modalities as a promising option to address the key challenges present in HCC management. More Details

Immune Checkpoint LAG3 and Its Ligand FGL1 in Cancer

LAG3 is the most promising immune checkpoint next to PD-1 and CTLA-4. High LAG3 and FGL1 expression boosts tumor growth by inhibiting the immune microenvironment. This review comprises four sections presenting the structure/expression, interaction, biological effects, and clinical application of LAG3/FGL1. D1 and D2 of LAG3 and FD of FGL1 are the LAG3-FGL1 interaction domains. LAG3 accumulates on the surface of lymphocytes in various tumors, but is also found in the cytoplasm in non-small cell lung cancer (NSCLC) cells. FGL1 is found in the cytoplasm in NSCLC cells and on the surface of breast cancer cells.
The LAG3-FGL1 interaction mechanism remains unclear, and the intracellular signals require elucidation. LAG3/FGL1 activity is associated with immune cell infiltration, proliferation, and secretion. Cytokine production is enhanced when LAG3/FGL1 are co-expressed with PD-1. IMP321 and relatlimab are promising monoclonal antibodies targeting LAG3 in melanoma. The clinical use of anti-FGL1 antibodies has not been reported. Finally, high FGL1 and LAG3 expression induces EGFR-TKI and gefitinib resistance, and anti-PD-1 therapy resistance, respectively. We present a comprehensive overview of the role of LAG3/FGL1 in cancer, suggesting novel anti-tumor therapy strategies.

Futility in Transcatheter Aortic Valve Implantation: A Search for Clarity

Although transcatheter aortic valve implantation (TAVI) has revolutionised the landscape of treatment for aortic stenosis, there exists a cohort of patients where TAVI is deemed futile. Among the pivotal high-risk trials, one-third to half of patients either died or received no symptomatic benefit from the procedure at 1 year. Futility of TAVI results in the unnecessary exposure of risk for patients and inefficient resource utilisation for healthcare services. Several cardiac and extra-cardiac conditions and frailty increase the risk of mortality despite TAVI. Among the survivors, these comorbidities can inhibit improvements in symptoms and quality of life.
However, certain conditions are reversible with TAVI (e.g. functional mitral regurgitation), attenuating the risk and improving outcomes. Quantification of disease severity, identification of reversible factors and a systematic evaluation of frailty can substantially improve risk stratification and outcomes. This review examines the contribution of pre-existing comorbidities towards futility in TAVI and suggests a systematic approach to guide patient evaluation.

Medical Imaging Biomarker Discovery and Integration Towards AI-Based Personalized Radiotherapy

Intensity-modulated radiation therapy (IMRT) has been used for high-accurate physical dose distribution sculpture and employed to modulate different dose levels into Gross Tumor Volume (GTV), Clinical Target Volume (CTV) and Planning Target Volume (PTV). GTV, CTV and PTV can be prescribed at different dose levels, however, there is an emphasis that their dose distributions need to be uniform, despite the fact that most types of tumour are heterogeneous.
With traditional radiomics and artificial intelligence (AI) techniques, we can identify biological target volume from functional images against conventional GTV derived from anatomical imaging. Functional imaging, such as multi parameter MRI and PET can be used to implement dose painting, which allows us to achieve dose escalation by increasing doses in certain areas that are therapy-resistant in the GTV and reducing doses in less aggressive areas.
In this review, we firstly discuss several quantitative functional imaging techniques including PET-CT and multi-parameter MRI. Furthermore, theoretical and experimental comparisons for dose painting by contours (DPBC) and dose painting by numbers (DPBN), along with outcome analysis after dose painting are provided. The state-of-the-art AI-based biomarker diagnosis techniques is reviewed. Finally, we conclude major challenges and future directions in AI-based biomarkers to improve cancer diagnosis and radiotherapy treatment.

Computational Methods for Single-Cell Imaging and Omics Data Integration

Integrating single cell omics and single cell imaging allows for a more effective characterisation of the underlying mechanisms that drive a phenotype at the tissue level, creating a comprehensive profile at the cellular level. Although the use of imaging data is well established in biomedical research, its primary application has been to observe phenotypes at the tissue or organ level, often using medical imaging techniques such as MRI, CT, and PET. These imaging technologies complement omics-based data in biomedical research because they are helpful for identifying associations between genotype and phenotype, along with functional changes occurring at the tissue level. Single cell imaging can act as an intermediary between these levels.
Meanwhile new technologies continue to arrive that can be used to interrogate the genome of single cells and its related omics datasets. As these two areas, single cell imaging and single cell omics, each advance independently with the development of novel techniques, the opportunity to integrate these data types becomes more and more attractive.
This review outlines some of the technologies and methods currently available for generating, processing, and analysing single-cell omics- and imaging data, and how they could be integrated to further our understanding of complex biological phenomena like ageing. We include an emphasis on machine learning algorithms because of their ability to identify complex patterns in large multidimensional data.

Procedure-Related Access Site Pain Multimodal Management following Percutaneous Cardiac Intervention: A Randomized Control Trial

Methods: 137 patients who underwent PCI procedure via radial artery were randomly assigned (1 : 1) to the control (CG, n = 68) and intervention (IG, n = 65) groups. IG received MPM (paracetamol, ibuprofen, and the arm physiotherapy), CG received pain medication “as needed.” Outcomes were assessed immediately after, 2, 12, 24, and 48 h, 1 week, and 1 and 3 months after PCI. The primary outcome was A-S pain prevalence and pain intensity numeric rating scale (NRS) 0-10.
Results: Results showed that A-S pain prevalence during the 3-month follow-up period was decreasing. Statistically significant difference between the groups (CG versus IG) was after 24 h (41.2% versus 18.5, p=0.005), 48 h (30.9% versus 1.5%, p ≤ 0.001), 1 week (25% versus 10.8%, p=0.042), 1 month (23.5% versus 7.7%, p=0.017) after the procedure. The mean of the highest pain intensity was after 2 h (IG-2.17 ± 2.07; CG-3.53 ± 2.69) and the lowest 3 months (IG-0.02 ± 0.12; CG-0.09 ± 0.45) after the procedure. A-S pain intensity mean scores were statistically significantly higher in CG during the follow-up period (Wilks’ λ = 0.84 F (7,125) = 3.37, p=0.002).

Gentodenz

19-DENZ-500 Gentaur Genprice 500 g 416 EUR

50ml TC Tubes, Conical, 440 units/box

04-5540150 Gentaur Genprice 440 units/box 85.2 EUR

GMP IL4, 50µg

04-GMP-HU-IL4-50UG Gentaur Genprice 50µg 579.6 EUR

SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE

04-GSB Gentaur Genprice
  • 144.00 EUR
  • 96.00 EUR
  • 1L
  • 500mL

rHu IL 2 , 3MIU

04-RHIL2-02F01 Gentaur Genprice 1 vial 298.8 EUR

rHu IL 2 , 3MIU , Lot 200908F02

04-RHIL2-08F02 Gentaur Genprice 1 vial 298.8 EUR

PRE-GMP rHu GM-CSF, Molgramostim-Leukoma

04-RHUGM-CSF-7A10 Gentaur Genprice 300 µg 462 EUR

Mouse Anti TNP Immunotoxicity

198-TNPG-1 Gentaur Genprice 100 µL 469.2 EUR

Rye Agar A

765-M1854-500G Gentaur Genprice 500 g 82 EUR

Rye Agar B

765-M1855-500G Gentaur Genprice 500 g 93 EUR

Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 Gentaur Genprice 192 Wells/kit 382 EUR

1-Step Polymorphs, Human Cell Separation

71-AN221725 Gentaur Genprice 1 238.8 EUR

QuantiChrom Hemoglobin Assay Kit

65-DIHB-250 Gentaur Genprice 250 tests 473 EUR

Malachite Green Phosphate Assay kit

65-POMG-25H Gentaur Genprice 2500 tests 333 EUR

Exo-Check Exosome Antibody Array

322-EXO-FBS-50A-1 Gentaur Genprice 100 µg 469.2 EUR

EasyNat Quattro

UC0104 Gentaur Genprice 4-module system 12000 EUR

EasyNat Octo

UC0108 Gentaur Genprice 8-module system 22000 EUR

Monkeypox Virus Real Time PCR Kit

ZD-0076-01 Gentaur Genprice 25 tests/kit Ask for price

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 Gentaur Genprice 25 tests/kit Ask for price

GMP Recombinant Human Interleukin-4 (IL-4)

GMPhuIL-4-50ug Gentaur Genprice 50ug 528 EUR

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 Bio Basic 10 mL 76.86 EUR

Rat G-Elongation Factor, Mitochondrial 2 (GFM2) ELISA Kit

abx391915-96tests Abbexa 96 tests 1093.2 EUR

G-Elongation Factor, Mitochondrial 2 (GFM2) Antibody

20-abx136068 Abbexa
  • 493.20 EUR
  • 710.40 EUR
  • 218.40 EUR
  • 376.80 EUR
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

G-Elongation Factor, Mitochondrial 1 (GFM1) Antibody

abx233430-100ug Abbexa 100 ug 577.2 EUR

G-Elongation Factor, Mitochondrial 2 (GFM2) Antibody

abx233431-100ug Abbexa 100 ug 610.8 EUR

G-Elongation Factor, Mitochondrial 2 (GFM2) Antibody

20-abx324860 Abbexa
  • 376.80 EUR
  • 292.80 EUR
  • 100 ug
  • 50 ug

G-Elongation Factor, Mitochondrial 2 (GFM2) Antibody

20-abx321190 Abbexa
  • 526.80 EUR
  • 393.60 EUR
  • 100 ul
  • 50 ul

G-Elongation Factor, Mitochondrial 2 (GFM2) Antibody

20-abx322012 Abbexa
  • 526.80 EUR
  • 393.60 EUR
  • 100 ul
  • 50 ul

cell tracking velocimetry as an economical and portable hematology analyzer

Anemia and iron deficiency continue to be the most prevalent nutritional disorders in the world, affecting billions of people in both developed and developing countries. The initial diagnosis of anemia is typically based on several markers, including red blood cell (RBC) count, hematocrit and total hemoglobin. Using modern hematology analyzers, erythrocyte parameters such as mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), etc. are also being used.
However, most of these commercially available analyzers pose several disadvantages: they are expensive instruments that require significant bench space and are heavy enough to limit their use to a specific lab and lead to a delay in results, making them less practical as a point-of-care instrument that can be used for swift clinical evaluation. Thus, there is a need for a portable and economical hematology analyzer that can be used at the point of need. In this work, we evaluated the performance of a system referred to as the cell tracking velocimetry (CTV) to measure several hematological parameters from fresh human blood obtained from healthy donors and from sickle cell disease subjects.
Our system, based on the paramagnetic behavior that deoxyhemoglobin or methemoglobin containing RBCs experience when suspended in water after applying a magnetic field, uses a combination of magnets and microfluidics and has the ability to track the movement of thousands of red cells in a short period of time. This allows us to measure not only traditional RBC indices but also novel parameters that are only available for analyzers that assess erythrocytes on a cell by cell basis.
As such, we report, for the first time, the use of our CTV as a hematology analyzer that is able to measure MCV, MCH, mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), the percentage of hypochromic cells (which is an indicator of insufficient marrow iron supply that reflects recent iron reduction), and the correlation coefficients between these metrics. Our initial results indicate that most of the parameters measured with CTV are within the normal range for healthy adults. Only the parameters related to the red cell volume (primarily MCV and RDW) were outside the normal range.
We observed significant discrepancies between the MCV measured by our technology (and also by an automated cell counter) and the manual method that calculates MCV through the hematocrit obtained by packed cell volume, which are attributed to the artifacts of plasma trapping and cell shrinkage. While there may be limitations for measuring MCV, this device offers a novel point of care instrument to provide rapid RBC parameters such as iron stores that are otherwise not rapidly available to the clinician. Thus, our CTV is a promising technology with the potential to be employed as an accurate, economical, portable and fast hematology analyzer after applying instrument-specific reference ranges or correction factors. More Details

Preparation of transparent photoluminescence smart window by integration of rare-earth aluminate nanoparticles into recycled polyethylene waste

Novel photoluminescent nanocomposite sheets were prepared for simple commercial manufacturing of transparent and luminous photochromic smart windows. Simple physical integration of lanthanide-doped strontium aluminium oxide (LdSAO) nanoparticles into recycled polyethylene (PE) waste introduced smart nanocomposite with persistent phosphorescence and photochromic properties. Because of the nanoparticle form of LdSAO is significant to develop transparent materials; LdSAO nanoparticles were well-dispersed in the polyethylene matrix. Both morphologies and chemical compositions of LdSAO nanoparticles and LdSAO-containing luminescent polyethylene sheets were investigated.
Both LdSAO-free and photoluminescent polyethylene sheets were colorless in regular daylight. Only LdSAO-containing polyethylene luminescent samples showed a brilliant green color under an UV supply and greenish-yellow color under darkness as verified by CIE Lab parameters. Both absorbance and emission bands were monitored at 377 and 436/517 nm, respectively.
For both photoluminescence spectroscopy and mechanical properties, the LdSAO-containing polyethylene luminescent sheets were compared to the LdSAO-free sample and found to have improved scratch resistance, UV protection, and superhydrophobic activity. Based on the added amount of LdSAO, photoluminescence, decay and lifetime spectral tests showed photochromic fluorescence and long-lasting phosphorescence characteristics. PELdSAO nanocomposite sheets displayed UV protection, photostability, hydrophobicity, excellent durability as compared to the blank LdSAO-free polyethylene sheet.

Financial development during COVID-19 pandemic: the role of coronavirus testing and functional labs

The outbreak of the SARS-CoV-2 virus in early 2020, known as COVID-19, spread to more than 200 countries and negatively affected the global economic output. Financial activities were primarily depressed, and investors were reluctant to start new financial investments while ongoing projects further declined due to the global lockdown to curb the disease.
  • This study analyzes the money supply reaction to the COVID-19 pandemic using a cross-sectional panel of 115 countries. The study used robust least square regression and innovation accounting techniques to get sound parameter estimates.
  • The results show that COVID-19 infected cases are the main contributing factor that obstructs financial activities and decrease money supply. In contrast, an increasing number of recovered cases and COVID-19 testing capabilities gave investors confidence to increase stock trade across countries.
  • The overall forecast trend shows that COVID-19 infected cases and recovered cases followed the U-shaped trend, while COVID-19 critical cases and reported deaths showed a decreasing trend. Finally, the money supply and testing capacity show a positive trend over a period.
  • The study concludes that financial development can be expanded by increasing the testing capacity and functional labs to identify suspected coronavirus cases globally.

Behavior of nitrogen and sulfur compounds in the rice husk pellet bioscrubber and its circulation water

In this study, pellet-type biofilter media was developed with rice husk and applied in a wet scrubber system for odor removal. The lab-scale bioscrubber system was operated for 200 days to evaluate odorous gas removal (i.e., NH3, H2S, methyl mercaptan, and dimethyl sulfide), and the removal mechanism of odor gases was studied by analyzing the behavior of nitrogen and sulfur compounds in circulation water of bioscrubber system. The rice husk pellets supplied the organic carbon source and phosphoric acid necessary for microbial growth, allowing the system to continue successfully for 200 days without any maintenance technology.
By analyzing the behavior of the nitrogen and sulfur compounds in the circulation water, we confirmed that the odor gas removal resulted from various mechanisms, including adsorption and biodegradation. Ammonia gas was absorbed by the rice husk pellets and accumulated in the circulation water as nitrite under conditions of sufficient alkalinity and above pH 7.
Conversely, when the alkalinity and pH decreased, nitrite was rapidly converted to nitrate. However, H2S gas was oxidized to sulfate and continuously accumulated in the circulation water regardless of the pH and alkalinity. In addition, it was confirmed that the decrease in nitrate in the bioscrubber system was due to heterotrophic denitrification by the organic carbon source supply and autotrophic denitrification by sulfur gas. During the operation of the rice husk pellet bioscrubber for 8 months, under low solubility condition, more than 99% of NH3 and H2S were removed and about 85% of methyl mercaptan (MM) and dimethyl sulfide (DMS) were removed.

LAB ALERT(R) FOUR CHANNEL TIMER AND CLO

HS24670 Scientific Laboratory Supplies EACH 40.8 EUR

LAB antibody

70R-2191 Fitzgerald 50 ug 560.4 EUR

LAB Antibody

ABD13143 Lifescience Market 100 ug 525.6 EUR

LAB Antibody

DF10355 Affbiotech 200ul 420 EUR

SLS Lab Pro PURA-Q Lab 20 Type 2

WAT2186 Scientific Laboratory Supplies EACH 7961.55 EUR

Ika Lab dancer

MIX4254 Scientific Laboratory Supplies EACH 454.8 EUR

Lab Pen Nalgene

6310-0010 Scientific Laboratory Supplies PK12 80.4 EUR

NATL/LAB Antibody

3838-100 Biovision each 379.2 EUR

NATL/LAB Antibody

3838-30T Biovision each 175.2 EUR

LAB (pY136) Antibody

abx216527-100ug Abbexa 100 ug 526.8 EUR

LAB Blocking Peptide

33R-6229 Fitzgerald 100 ug 216 EUR

LAB Blocking Peptide

DF10355-BP Affbiotech 1mg 234 EUR

PhotoFlex Lab Station

WAT5107 Scientific Laboratory Supplies EACH 648.88 EUR

Lab Marker Pen Blue

LAB2902 Scientific Laboratory Supplies PK10 90.09 EUR

Lab Marker Pen Green

LAB2904 Scientific Laboratory Supplies PK10 90.09 EUR

Lab Jack 50x40mm Blue

JAC0998 Scientific Laboratory Supplies EACH 176.4 EUR

Blue Lab Tape 55Mx19mm

7560-002 Scientific Laboratory Supplies EACH 28.8 EUR

Lab Jack 100x100mm Blue

JAC1000 Scientific Laboratory Supplies EACH 166.8 EUR

Lab Jack 150x150mm Blue

JAC1004 Scientific Laboratory Supplies EACH 220.8 EUR

Lab Jack 200x200mm Blue

JAC1008 Scientific Laboratory Supplies EACH 277.2 EUR

Lab Jack 200x230mm Blue

JAC1012 Scientific Laboratory Supplies EACH 337.2 EUR

Lab Jack 320x260mm Blue

JAC1013 Scientific Laboratory Supplies EACH 752.4 EUR

Bangs Lab Bead Solution

SOLN1-1000 Bangs Laboratories 1000 ML 186.07 EUR

Bangs Lab Bead Solution

SOLN1-2000 Bangs Laboratories 2000 ML 255.24 EUR

 

 

Direct and Indirect Chemiluminescence: Reactions, Mechanisms and Challenges

The phenomenon can take place both in natural and artificial chemical systems and it has been utilized in a variety of applications. In this review, we aim to revisit some of the latest CL applications based on direct and indirect production modes. The characteristics of the chemical reactions and the underpinning CL mechanisms are thoroughly discussed in view of studies from the very recent bibliography.
Different methodologies aiming at higher CL efficiencies are summarized and presented in detail, including CL type and scaffolds used in each study. The CL role in the development of efficient therapeutic platforms is also discussed in relation to the Reactive Oxygen Species (ROS) and singlet oxygen (1O2) produced, as final products.
Moreover, recent research results from our team are included regarding the behavior of commonly used photosensitizers upon chemical activation under  CL conditions. The CL prospects in imaging, biomimetic organic and radical chemistry, and therapeutics are critically presented in respect to the persisting challenges and limitations of the existing strategies to date.

A Novel Brighter Bioluminescent Fusion Protein Based on ZZ Domain and Amydetes vivianii Firefly Luciferase for Immunoassays

Immunoassays are widely used for detection of antibodies against specific antigens in diagnosis, as well as in electrophoretic techniques such as Western Blotting. They usually rely on colorimetric, fluorescent or chemiluminescent methods for detection. Whereas the chemiluminescence methods are more sensitive and widely used, they usually suffer of fast luminescence decay. Here we constructed a novel bioluminescent fusion protein based on the N-terminal ZZ portion of protein A and the brighter green-blue emitting Amydetes vivianii firefly luciferase.
In the presence of D-luciferin/ATP assay solution, the new fusion protein displays higher bioluminescence activity, is very thermostable and produces a sustained emission (t1/2 > 30 min). In dot blots, we could successfully detect rabbit IgG against firefly luciferases, Limpet Haemocyanin, and SARS-CoV-2 Nucleoprotein (1-250 ng), as well as the antigen bound antibodies using either CCD imaging, and even photography using smartphones. Using CCD imaging, we could detect up to 100 pg of SARS-CoV-2 Nucleoprotein.
Using this system, we could also successfully detect firefly luciferase and SARS-CoV-2 nucleoprotein in Western Blots (5-250 ng). Comparatively, the new fusion protein displays slightly higher and more sustained luminescent signal when compared to commercial HRP-labeled secondary antibodies, constituting a novel promising alternative for Western Blotting and immunoassays.

Long-Lasting Luminol Chemiluminescence Emission with 1,10-Phenanthroline-2,9-dicarboxylic Acid Copper(II) Complex on Paper

As most of the known systems are flashtype, long-lasting chemiluminescence (CL) emissions are extremely needed for the application of cold light sources, accurate CL quantitative analysis, and biological mapping. In this work, the flashtype system of luminol was altered to a long lasting CL system just because of the paper substrate. The Cu(II)-based organic complex was loaded on the paper surface, which can trigger luminol-H2O2 to produce a long lasting CL emission for over 30 min.
By using 1,10-phenanthroline-2,9-dicarboxylic acid (PDA) as the ligand, a hexacoordinated Cu(II)-based organic complex was synthesized by the simple freeze-drying method. It is interesting that the complex morphology can be controlled by adding different amounts of water in the synthesizing procedure. The complex with a certain size can be definitely trapped in the pores of the cellulose.
  • Then, slow diffusion, which can be attributed to the long lasting CL emission, was produced. With the high catalytic activity of the complex, reactive oxygen species from H2O2 was generated and was responsible for the high CL intensity.
  • By using the paper substrate, the flash-type luminol system can be easily transferred to the long-duration CL system without any extra reagent.
  • This long-lasting emission system was used for hydrogen sulfide detection by the CL imaging method.
  • This paper-based sensor has great potential for CL imaging in the clinical field in the future.

Insight into the Ozone-Assisted Low-Temperature Combustion of Dimethyl Ether by Means of Stabilized Cool Flames

The low-temperature combustion kinetics of dimethyl ether (DME) were studied by means of stabilized cool flames in a heated stagnation plate burner configuration using ozone-seeded premixed flows of DME/O2. Direct imaging of CH2O* chemiluminescence and laser-induced fluorescence of CH2O were used to determine the flame front positions in a wide range of lean and ultra-lean equivalence ratios and ozone concentrations for two strain rates.
The temperature and species mole fraction profiles along the flame were measured by coupling thermocouples, gas chromatography, micro-chromatography, and quadrupole mass spectrometry analysis. A new kinetic model was built on the basis of the Aramco 1.3 model, coupled with a validated submechanism of O3 chemistry, and was updated to improve the agreement with the obtained experimental results and experimental data available in the literature.
The main results show the efficiency of the tested model to predict the flame front position and temperature in every tested condition, as well as the importance of reactions typical of atmospheric chemistry in the prediction of cool flame occurrence. The agreement on the fuel and major products is overall good, except for methanol, highlighting some missing kinetic pathways for the DME/O2/O3 system, possibly linked to the direct addition of atomic oxygen on the fuel radical, modifying the product distribution after the cool flame.

Advanced image analysis-based evaluation of protein antibody microarray chemiluminescence signal improves glioma type identification by blood serum proteins concentrations

Background and objective: Gliomas are the most common brain tumors usually classified as benign low-grade or aggressive high-grade glioma. One of the promising possibilities of glioma diagnostics and tumor type identification could be based on concentration measurements of glioma secreted proteins in the blood.
However, several published approaches of quantitative proteomic analysis emphasize limits of one single protein to be used as biomarker of these types of tumors. Simultaneous multi-protein concentrations analysis giving antibody array-based methods suffer from poor measurement accuracy due to technical limitations of imaging systems.
Methods: We applied Principal Component Analysis (PCA) for series of repeated antibody array chemiluminescence images to extract the component representing relative values of protein concentrations, free from zero-mean noise and uneven background illumination – main factors corrupting evaluation result.
Results: The proposed method increased accuracy of protein concentration estimates at least 2-fold. Decision tree classifier applied to the relative concentration values of three proteins TIMP-1, PAI-1 and NCAM-1 estimated by proposed image analysis method effectively distinguished between low-grade glioma, high-grade glioma and healthy control subjects showing validation accuracy of 74.9% with the highest positive predictive value of 81.2% for high-grade glioma and 57.1% for low-grade glioma cases.

SuperBrite? ELISA HRP Chemiluminescence Substrate Kit

K4005-500 Biovision each 216 EUR

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K002M T-Pro Biotechnology 250ml*2/Kit 244.8 EUR

T-Pro LumiFast Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K002S T-Pro Biotechnology 100ml*2/Kit 182.4 EUR

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K004M T-Pro Biotechnology 250ml*2/Kit 286.8 EUR

T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

JT96-K004S T-Pro Biotechnology 100ml*2/Kit 204 EUR

GloBrite chemiluminescence reagent kit for western blotting 200 mL

GLB1 Detroit R&D 200 mL 174 EUR

GloBrite chemiluminescence reagent kit for western blotting 500 mL

GLB2 Detroit R&D 500 mL 280.8 EUR

High-Sensitive ECL Chemiluminescence Detection Kit (Ready-to-Use)

E412-01 Vazyme 2 × 50 ml 180 EUR

High-Sensitive ECL Chemiluminescence Detection Kit (Ready-to-Use)

E412-02 Vazyme 250 ml 393.6 EUR

Spike S1 (B.1.1.7 Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit

78154 BPS Bioscience 96 rxns. 995 EUR

Spike S1 RBD (B.1.351 Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit

78151 BPS Bioscience 96 rxns. 995 EUR

Spike RBD (B.1.1.7 Variant) (N501Y) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit

78140 BPS Bioscience 96 rxns. 995 EUR

Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit

78357 BPS Bioscience 96 rxns. 995 EUR

Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Chemiluminescence Assay Kit

78369 BPS Bioscience 96 rxns. 995 EUR

PolyTek HRP Anti-Rabbit Polymerized Imaging System

PIP080 ScyTek Laboratories 1 kit(s) 187.2 EUR

PolyTek Anti-Mouse (DAB) Polymerized HRP Imaging System

PIR080 ScyTek Laboratories 70 Slides 187.2 EUR

EzScope 101 Live Cell Imaging System with 10x objective lens

COU2050 Scientific Laboratory Supplies EACH 5950.92 EUR

FTO Chemiluminescent Assay Kit

79344 BPS Bioscience 96 rxns. 765 EUR

UTX Chemiluminescent Assay Kit

50615 BPS Bioscience 96 rxns. 715 EUR

G9a Chemiluminescent Assay Kit

52001L BPS Bioscience 96 rxns. 750 EUR

NSD3 Chemiluminescent Assay Kit

79358 BPS Bioscience 384 rxns. 1950 EUR

NSD2 Chemiluminescent Assay Kit

79359 BPS Bioscience 384 rxns. 1100 EUR

P300 Chemiluminescent Assay Kit

79705 BPS Bioscience 96 rxns. 465 EUR

EZH1 Chemiluminescent Assay Kit

52990 BPS Bioscience 384 rxns. 4120 EUR

NSD2 Chemiluminescent Assay Kit

53009 BPS Bioscience 96 rxns. 750 EUR

Rotary cell culture system (RCCS)

Rotary cell culture system (RCCS)

RCCS is a unique bioreactor technology that produces 3D cultures based on the principle of clinorotation, which is defined as the abolition of gravitational force by slow rotation around one or two axes.

RCCS is a dynamic system that suspends cells without mechanical damage and allows cells to easily aggregate into a 3D spheroid with a sufficient supply of nutrients and oxygen. RCCS can also be used for co-cultivation of several types of cell cultures, cells can be cultured with or without the use of various scaffolds.

It is the first bioreactor system designed to simultaneously integrate the ability to co-cultivate cells and features low shear (and consequently low turbulence) and high nutrient transfer to cells. Together, these properties promote spheroid formation and cell proliferation in three-dimensional spheroids.

Two types of bioreactors: disposable 3D bioreactors and autoclavable 3D bioreactors